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biotinylated mgfrα 2  (R&D Systems)


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    R&D Systems biotinylated mgfrα 2
    Biotinylated Mgfrα 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+mgfr%CE%B1+2/Mouse+GFR+alpha-2%2FGDNF+R+alpha-2+Biotinylated+Antibody/pmc06028518-237-6-9
    Average 90 stars, based on 2 article reviews
    biotinylated mgfrα 2 - by Bioz Stars, 2026-10
    90/100 stars

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    Incubation:

    Article Title: Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis
    Article Snippet: Lineage-positive cells were removed by staining with purified antibodies CD19(6D5), CD8a(53–6.7), CD4(GK1.5), Gr1(RB6-8C5), Mac1(M1/70), and Ter119(TER-119), followed by negative selection by using Dynabeads Sheep Anti–Rat IgG (11035; Invitrogen). .. Remaining cells were next incubated with biotinylated mGFRα-2 (BAF429; R&D Systems) and further stained with lineage-PECy5 Gr1(RB6-8C5), Mac1(M1/70), Ter119(TER-119), CD11c(N418), NK1.1(PK136), CD3 ε(145−2c11), CD19-PECy7 (ID3; eBioscience), B220-Alexa Flour 700 (RA3-6B2), and Streptavidin-Brilliant Violet (BV) 605. .. Lin − CD19 − B220 + GFRA2 + and Lin − CD19 − B220 + GFRA2 − cells were sorted on BD FACSAriaIII, and 2,000 cells per 150 µl 1% PBS/FCS were transplanted by intravenous injection via tail vein to 9–11-wk-old preconditioned (2.25 Gray) NOD.Cg- Prkdc scid Il2rg tm1WjI /SzJ (NSG) mice.

    Staining:

    Article Title: Dissection of progenitor compartments resolves developmental trajectories in B-lymphopoiesis
    Article Snippet: Lineage-positive cells were removed by staining with purified antibodies CD19(6D5), CD8a(53–6.7), CD4(GK1.5), Gr1(RB6-8C5), Mac1(M1/70), and Ter119(TER-119), followed by negative selection by using Dynabeads Sheep Anti–Rat IgG (11035; Invitrogen). .. Remaining cells were next incubated with biotinylated mGFRα-2 (BAF429; R&D Systems) and further stained with lineage-PECy5 Gr1(RB6-8C5), Mac1(M1/70), Ter119(TER-119), CD11c(N418), NK1.1(PK136), CD3 ε(145−2c11), CD19-PECy7 (ID3; eBioscience), B220-Alexa Flour 700 (RA3-6B2), and Streptavidin-Brilliant Violet (BV) 605. .. Lin − CD19 − B220 + GFRA2 + and Lin − CD19 − B220 + GFRA2 − cells were sorted on BD FACSAriaIII, and 2,000 cells per 150 µl 1% PBS/FCS were transplanted by intravenous injection via tail vein to 9–11-wk-old preconditioned (2.25 Gray) NOD.Cg- Prkdc scid Il2rg tm1WjI /SzJ (NSG) mice.



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    biotinylated mgfrα 2  (R&D Systems)
    90
    R&D Systems biotinylated mgfrα 2
    Biotinylated Mgfrα 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+mgfr%CE%B1+2/Mouse+GFR+alpha-2%2FGDNF+R+alpha-2+Biotinylated+Antibody/pmc06028518-237-6-9
    Average 90 stars, based on 1 article reviews
    biotinylated mgfrα 2 - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

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